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Kumar, H. D. Mohan
- Genetic Diversity in Commercial Varieties of Chilli as Revealed by RAPD Method
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Authors
Affiliations
1 Dept. of Biotech., G.M. Instt. of Technol., Davangere, IN
2 The Oxford College of Engg., Bangalore, Karnataka, IN
3 Dept. of Plant Breeding & Genetics, Agri. Res. Station, Univ. Agri. Sci., Devihosur, Haveri, Karnataka, IN
1 Dept. of Biotech., G.M. Instt. of Technol., Davangere, IN
2 The Oxford College of Engg., Bangalore, Karnataka, IN
3 Dept. of Plant Breeding & Genetics, Agri. Res. Station, Univ. Agri. Sci., Devihosur, Haveri, Karnataka, IN
Source
Indian Journal of Science and Technology, Vol 2, No 4 (2009), Pagination: 91-94Abstract
The study aimed at investigating the genetic diversity of ten different commercial varieties of chilli using random amplified polymorphic DNA (RAPD) analysis. The DNA extracted by CTAB method from the leaf sample was amplified by successive PCR cycles. Using 12 different primers, the amplified DNA solution was subjected to agarose gel electrophoresis. Around 500 different bands were observed under UV light. Seven were polymorphic for a specific primer and can be used as differential markers. This effort can serve determining genetic diversity among ten commercial varieties of chilli in India.Keywords
Chilli, RAPD, Molecular Markers, RFLPFull Text
References
- Agwana CO, Lashermes P, Trouslot P, Combes M and Charrier A (1997) Identification of RAPD markers for resistance to coffee berry disease, Colletotrichum kahawae, in arabica coffee. Euphytica. 97, 241-248.
- Alahakoon PW, Brown AE and Sreenivasaprasad S (1994) Cross-infection potential of genetic groups of Colletotrichum gloepsporioides on tropical fruits. Physiol. Mol. Plant Pathol. 44, 93-103.
- Amusa NA, Ashaye OA, Oladapo MO and Oni MO (2005) Guava fruit anthracnose and the effects on its nutritional and market values in Ibadan, Nigeria. World J. Agri. Sci. 1 (2), 169-172.
- Angelo Karakousis and Peter Langridge (2003) A highthroughput plant DNA extraction method for marker analysis. Plant Mol. Biol. Reporter. 21, 95a-95f.
- Backman PA, Landschoot PJ and Huff DR (1999) Variation in pathogenicity, morphology and RAPD marker profiles in Colletotrichum graminicolo fromTurf grasses. Crop Sci. 39, 1129-1135.
- Eyal Blum and Ilan Paran (2003) Molecular mapping of capsaicinoid biosynthesis gene. Theor. Appl. Genet. 108, 79-86.
- Eyal Blum and Kede Liu (2002) Molecular mapping of C locus for presence of pungency i capsicum, Department of plant breeding, NRC research press web site.
- Freeman S, Katan T and Shabi E (1998) Characterization of Colletotrichum species responsible for anthracnose diseases of various fruits. Plant Disease. 82, 596-605.
- Gopinath K, Radhakrishnan NV and Jayaral J (2006) Effect of propiconazole and difenoconazole on the control of anthracnose of chilli fruit caused by Colletotrichum capsici. Crop Protect. 25, 1024-1031.
- Hong JK and Hwang BK (1998) Influence of inoculum density, wetness duration, plant age, inoculation method, Colletotrichum coccodes. Plant Disease. 82, 1079-1083.
- Jordi B and Carmen M (1998) Determination of F1 hybrid seed purity in pepper using PCR- based markers. Euphytica. 103, 223-226.
- Joseph A (1993) A taxocological review capsaicinoid (oleorecin of capsicums). Ruddick Hazardous Product Section, Environmental Health, Directorate Health and Welfare, Canada.
- Martínez-Culebras PV, Barrio E, García MD and Querol A (2000) Identification of Colletotrichum
- species responsible for anthracnose of strawberry based on the internal transcribed spacers of the ribosomal region. FEMS Microbiol. Letters. 189 (1), 97-101.